Cytogenetics laboratory

Medical Director:       John Reinartz, MD
Technical Advisor:    Bill Wyatt, CG (ASCP)CM

The Allina Health Cytogenetics Laboratory provides comprehensive testing services to detect chromosomal abnormalities for congenital disorders, prenatal diagnosis, hematologic malignancies and solid tumors. We are a full service Cytogenetics Laboratory with expertise in chromosome analysis and fluorescence in situ hybridization (FISH). All of our technical staff are certified in Cytogenetics. Our highly trained professional staff provides accurate, high quality results with excellent turn-around-times and exceptional customer service. We also partner with genetic counselors and pathologists to support the needs of our patients and providers.

For information on specimen collection, oncology and congenital requisitions, see the resource section at the bottom of this webpage.

For more information, contact the Cytogenetics Laboratory at (612) 863-4541 or Client Services at (612) 863-4678.

 

TP53 metaphase - A normal DAPI (blue) stained metaphase cell with a red TP53 gene    

FISH signal and green chromosome 17 centromere signal on each chromosome 17.

Hours

Mo - Fr:  8am - 5pm
Sa:         8am - 4pm
Su:        Closed; on-call until noon for  FISH PML/RARA only
 
 

Test Lists

 

CONGENITAL CYTOGENETIC TESTS

Chromosome analysis studies

Microarray (CMA)

  • Currently a sendout test referred to Mayo Clinic Laboratories
  • Prenatal specimens require maternal and paternal blood specimens collected in EDTA

Combination test – Limited chromosome analysis study plus CMA analysis

FLUORESCENT IN SITU HYBRIDIZATION (FISH) TESTS
Aneuploidy Perinatal X/Y/13/18/21 panel
Aneuploidy X/Y/18 ONLY panel
Aneuploidy 13/21 ONLY panel
DiGeorge/VCFS/CATCH22 (DGS) 22q11.2

OTHER CONGENITAL SERVICES

Tissue culture 
Follow-up parental/family studies
Sendout/referral testing

ONCOLOGY CYTOGENETIC TESTS

Chromosome analysis studies

5q31 deletion / Monosomy 5 (-5)
6q deletion
7q31 deletion / Monosomy 7 (-7)
Trisomy 8 (+8)
Trisomy 12 (+12)
13q14 deletion
20q12 deletion
Aneuploidy 4/10
ALK (Lymphoma) 2p23
API2/MALT1 t(11;18)
ATM deletion 11q22.3
BCL2 18q21
BCL2/IGH t(14;18)
BCL6 3q27
BCR/ABL1 t(9;22)
CBFB 16q22
CCND1/IGH t(11;14)
CCND3/IGH t(6;14)
CDKN2C (P18)/CKS1B 1p32.3/1q21
CHIC2/FIP1L1/PDGFRA 4q12
ETV6 (TEL) 12p13
ETV6/RUNX1 (TEL/AML1) t(12;21)
EWSR1 22q12
FGFR1 8p12
FGFR3/IGH t(4;14)
IGH 14q32
IGH/MAF t(14;16)
IGH/MAFB t(14;20)
IGH/MALT1 t(14;18)
MALT1 18q21
MLL 11q23
MYC 8q24
MYC/IGH t(8;14)
P16 (CDKN2A) deletion 9p21
PDGFRB 5q32
PML/RARA t(15;17)
RUNX1T1/RUNX1 (AML1/ETO) t(8;21)
SYT (SS18) 18q11.2
TCR (TRA/D) 14q11
TCRB 7q34
TCL1 14q32
TP53 deletion 17p13.1

ONCOLOGY FISH PANELS

B Cell ALL panel

  • BCR/ABL1 [t(9;22)], ETV6/RUNX1 [t(12;21)], MLL (11q23), Aneuploidy 4/10
    • Reflex to Mayo Clinic Laboratories for Ph+-like B-cell ALL FISH panel

T Cell ALL panel

  • BCR/ABL1 [t(9;22)], P16 (CDKN2A) 9p21
    • Reflex to: TCR (TRA/D) 14q11.2, TCRB 7q34, TCL1 14q32

AML panel

  • BCR/ABL1 [t(9;22)], RUNX1T1/RUNX1 [t(8;21)], MLL (11q23), CBFB (16q22), 5q31 deletion/ -5, 7q31 deletion/ -7

CLL panels

  • Full panel: 6q deletion, ATM deletion (11q22.3), Trisomy 12, 13q14 deletion, TP53 deletion (17p13.1), CCND1/IGH [t(11;14)]
  • Prognositc panel: ATM deletion (11q22.3), Trisomy 12, 13q14 deletion, TP53 deletion
  • Progressive panel: ATM deletion (11q22.3), TP53 deletion

Eosinophilia panel

  • BCR/ABL1 [t(9;22)], CHIC2-PDGFRA (4q12), PDGFRB (5q32), FGFR1 (8p12)

High grade Lymphoma panel

  • MYC (8q24), IGH/BCL2 [t(14;18)], BCL6 (3q27)

MDS panel

  • 5q31 deletion / -5, 7q31 deletion / -7, Trisomy 8, MLL (11q23), 20q12 deletion

Plasma cell dyscrasia panel (with plasma cell enrichment)

  • CDKN2C/CKS1B (1p/q), TP53 (17p13.1), Aneuploidy 5, 9, 15
  • IGH (14q32) with reflex CCND1/IGH [t(11;14)], FGFR3/IGH [t(4;14)] IGH/MAF [t(14;16)], CCND3/IGH [t(6;14)], IGH/MAFB [t(14;20)]

ONCOLOGY MORPHOMETRIC/PATHOLOGY FISH TESTS
ALK Non-small cell lung cancer 2p23
CHOP (DDIT3) Myxoid liposarcoma 12q13
EWSR1 Ewing Sarcoma 22q12
FOXO1 (FKHR) Rhabdomyosarcoma 13q14
FUS Fibromixoid sarcoma 16p11.2
HER2/neu Breast and gastric cancer 17q12
MDM2 Liposarcoma 12q15
Oligodendroglioma panel 1p36/19q13
ROS1 Non-small cell lung cancer 6q22
SYT (SS18) synovial sarcoma 18q11.2
Urine bladder cancer aneuploidy P16, 3,7,17
 
 
 

Resources