Tropheryma whipplei, molecular detection, PCR, varies-994

Test info

  
Tropheryma whipplei, molecular detection, PCR, varies
  
994
  
LAB994
  
MSO
  
Whipple's Disease
  

Aids in the diagnosis of Whipple disease, especially for identifying inconclusive or suspicious cases

Specimen

  
Synovial fluid
  
0.5 mL
  
  • Container/tube:
    • Preferred: Lavender top (EDTA)
    • Acceptable: Navy (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container
  
  1. Place a minimum of 0.5 mL of fluid into a Lavender (EDTA) tube 
  2. Mix by gentle inversion
  3. Send specimen in original tube (preferred)
  
  
Fresh tissue or biopsy
  
Minimum 5 mm(3)
  

Fresh tissue or biopsy

  • Sources: Small intestine tissue (duodenum, ileum, or jejunum), lymph node, other visceral tissue, synovial tissue, gastrointestinal tissue, heart valve, or brain
  • Specimen volume: Entire collection or 5 mm3
  1. Collect fresh tissue specimen
  2. Submit tissue only, do not add fluid to tissue

Fluid

  • Sources: Cerebrospinal or ocular (eg, vitreous humor)
  • Specimen volume: 0.5 mL
  

Sterile vial/container

  

Refrigerated (preferred): <7 days

Frozen: <7 days

  
1 week
  
  • Slides
  • Bone marrow
  • PCR testing cannot be added on to a sample that has been opened and/or used for other testing. If the sample has already been used for testing, a new specimen will need to be collected.

Performance

  
Mayo Clinic Laboratories (TWRP): R-NX
  
Mo - Fr
  
2 - 7 days
  

Real-time Polymerase Chain Reaction (PCR)

Clinical and Interpretive info

  
  • A positive result indicates the presence of Tropheryma whipplei DNA.
  • A negative result indicates the absence of detectable T whipplei DNA, but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of T whipplei DNA in quantities less than the limit of detection of the assay.

Billing

  
87798

Tracking

  
11/06/2019
  
01/04/2024
  
01/04/2024