FOXO1 by FISH-4280J

Test info

  
FOXO1 by FISH
  
4280J
  
LAB4280J - CYTOGENETICS MALIGNANT TISSUE STUDIES
  
CG FOXO1
  
Alveolar rhabdomyosarcoma
FOXO1
13q14
  

Chromosomal rearrangements involving the FOXO1 gene (located at G-band region 13q14) are recurrent findings in cases of alveolar rhabdomyosarcoma. Two translocations, t(2;13)(q35;q14) and t(1;13)(p36;q14), juxtapose portions of the PAX3 or PAX7 gene (on chromosomes 2 or 1, respectively) with portions of the FOXO1 gene.

Specimen

  
Tumor tissue (solid tumor or other)
  

Sterile container filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline.

  
5 mm (3)
  

Specimens should be immersed in sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline within one (1) hour of procedure

  
Tumor tissue FFPE block
Tumor tissue FFPE or slides
  
Block
Slides: 2 plus one H & E stained slide
  
  1. Fix the tissue in 10% formalin for 6 - 72 hours.
  2. Prepare a Formalin-fixed, paraffin-embedded (FFPE) tissue block

Either the entire block, or two (2) 5 micron cuts mounted on positively charged slides and one (1) H&E stained slide can be submitted for testing

If the entire block is submitted, an H&E will be cut and stained, and assessed by an HPA pathologist.

  

FFPE tissue block or

Tissue section mounted on charged, unstained slides

  
  • Cytogenetics oncology requisition.
  

Ambient

  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Improper storage
  • Leaking container
  • Slides broken beyond repair

Performance

  
AHL - Cytogenetics: X
  
As needed
  
5 days
  

Fluorescence in situ hybridization (FISH)

Clinical and Interpretive info

  

Positive for rearrangement: >1.5% of 200 nuclei

Negative for rearrangement: ≤1.5% of 200 nuclei

Billing

  
88377 – Quantitative Morphometric FISH

This test includes a charge for application of the 2 FISH probes and professional interpretation of the results. If no cells are available for analysis, no analysis charges will be incurred.

Tracking

  
03/11/2021
  
04/06/2021