• All IHC stains will include a positive control tissue

• Highly specific marker for identifying melanoma
• Tyrosinase will not cross react with melanophages, which may obscure the identification of dermal melanocytic cells
• This marker is 100% specific and >80% sensitive for non-spindle cell type melanomas<
• Tyrosinase is 100% specific and 85% sensitive for amelanotic melanomas
• This marker can help identify cellular blue nevi that may be S100 negative
• Tyrosinase is a very specific marker for identifying malignant melanoma in sentinel lymph nodes

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Tyrosinase is an enzyme, that catalyzes the reaction that forms melanin
• This antigen is a highly specific marker for melanocytic differentiation, and is only present in melanin producing cells

Staining pattern

• Cytoplasmic granular staining (a red chromogen is used)

References

1. Kaufmann O et al: Tyrosinase, melan-A, and KBA62 as markers for the immunohistochemical identification of metastatic amelanotic melanomas on paraffin sections. Mod Pathol 1998; 11:740-746.
2. Orchard GE. Comparison of immunohistochemical labeling of melanocyte differentiation antibodies melan-A, tyrosinase, and HMB-45 with NK1/C3 and S100 protein in the evaluation of benign nevi and malignant melanoma. Histochem J 2000:32:475-481.