Rule out Crohn's disease and ulcerative colitis.
SST - spin
Red - spin and separate.
Serum separator (SST) tube
LabCorp 12mL plastic transport tube labelled as serum
Specimen should be free of bacterial contamination, hemolysis, and lipemia.
Atypical ANCA: Indirect fluorescent antibody (IFA)
Saccharomyces cerevisiae, IgA and IgG: Enzyme immunoassay (EIA)
Saccharomyces cerevisiae, IgA: negative: < 20.0
Saccharomyces cerevisiae, IgG:
Equivocal: 20.1 - 24.9
positive: ≥ 25.0
Atypical antineutrophil cytoplasmic antibodies: negative: < 1:20
IgA and IgG antibody testing for S. cerevisiae is useful adjunct testing for differentiating Crohn's disease and ulcerative colitis. Nearly 80% of Crohn's disease patients are positive for either IgA or IgG. In ulcerative colitis, less than 15% are positive for IgG, and less than 2% are positive for IgA. Fewer than 5% of healthy controls are positive for either IgG or IgA antibody, and no healthy controls had antibody for both. The atypical ANCA pattern has been observed in a significant percentage of patients with ulcerative colitis, primary sclerosing cholangitis, and autoimmune hepatitis.