SATB2 by IHC

Alphabetical Test listing

SATB2 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

SATB2 by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Tests included:

All IHC stains will include a positive control tissue

Useful for:

SATB2 can be used in the panel (CK7/CK20/CDX2/CADH17) For use in cancer of unknown primary to differentiate colorectal carcinoma from upper gastrointestinal and pancreatobiliary origin
SATB2 can be used differentiate help differentiate ovarian metastasis from colorectal and appendiceal sites from other metastatic sites (upper GI and pancreatobiliary and endocervical)
SATB2 can be used differentiate help differentiate ovarian metastasis from colorectal and appendiceal origin from primary ovarian mucinous and ovarian endometrioid tumors
SATB2 is positive in 80-97% of primary and metastatic colorectal adenocarcinomas and colonic medullary carcinomas., which can be negative for CK20 and CDX2¹
In one study of ovarian metastases of extra ovarian origin, SATB2 was expressed by 75% of colorectal adenocarcinomas, 80% of low grade appendiceal neoplasms and 100% of high grade appendiceal adenocarcinomas²
A minority of ovarian mucinous tumors with teratomatous elements will demonstrates SATB2 positivity in the epithelium ²
SATB2 positivity can be seen in a minority of carcinomas of upper GI, pancreatobiliary, pulmonary and urothelial origin and can also be seen in a minority of carcinoid tumors
SATB2 is positive in a majority of osteosarcomas

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin embedded tissue block

Transport container:

Formalin-fixed, paraffin embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

SATB2 is a DNA binding protein involved in the regulation of transcription and chromatin remodeling in osteoblastic and neuronal differentiation and is involved in skeletal development.

Staining pattern

Nuclear staining only
Positive staining occurs in normal colonic and rectal epithelium, normal appendix, neurons in the frontal cortex and hippocampus, normal and neoplastic osteoblastic tissues. Gynecologic tissues (ovarian, endometrium, cervical) are negative. Weak positivity can be seen in the epididymis, seminiferous tubules and in some lymphoid cells

References

Dragomir A, De Wit M, Johansson C, Uhlen M et al. The role of SATB2 as a diagnostic marker for tumors of colorectal origin. AJCP 2014;141:630-638.
Moh M, Krings G, Ates D, Aysal A, Kim G et al. SATB2 expression distinguishes ovarian metastases of colorectal and appendiceal origin from primary ovarian tumors of mucinous or endometrioid type AJSP 2016;40(3):419-432.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

10/29/2017

Revised date:

10/17/2018

Review date:

01/12/2024