Kleihauer (Fetal cell)

Alphabetical Test listing

Kleihauer (Fetal cell)-457

Test name:

Test number:

457

Excellian order number:

85460.0

Abbreviation:

KLE

Alternate names:

Fetal hemoglobin stain
Fetal RBC enumeration

Useful for:

Determine the present of fetal cells in maternal peripheral blood (screen and quantitate fetal maternal hemorrhage)

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

4.0 mL

Minimum volume:

3.0 mL

Collection instructions:

Immediatley following collection, mix sample by inverting 8 - 10 times to prevent clotting

Transport container:

Lavender (EDTA), 4mL

Required forms/information:

Excellian clients must complete the order level information.
All other clients submit a completed requisition along with the specimen

Transport and stability:

Refrigerated (within 6 hours of collection) - 72 hours

Specimen retention time:

14 days

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper blood/anticoagulant ratio
Delay in transport
Improper storage
Inappropriate timing of collection
Interfering substances
Leaking container

Performing lab:

Mo - Fr: AHL - Flow Cytometry
Sa - Su: Hematology/Special Hematology (Rh Negative patients only)
Note: Specimens received in the testing department after 11 am will be held for testing the next day.

Days set up:

Daily

TAT:

1 day

Method:

Kleihauer stain - Acid elution test

Flow Cytometry - anti-Fetal Hgb monoclonal antibody staining

Reference ranges:

Kleihauer stain - None detected

Flow Cytometry - ≤ 0.03%

Clinical information:

May be ordered STAT if maternal trauma is suspected of causing fetal maternal hemorrhage

All results will be called to the number provided

Tubes and markers:

Tube: FH (10C)

Markers: Fetal Hgb

CPT codes:

85460 - Kleihauer Stain
or
88184 - Flow Cytometry

Date created:

11/30/2001

Revised date:

11/12/2020

Review date:

01/03/2023