Tropheryma whipplei, molecular detection, PCR, varies

Alphabetical Test listing

Tropheryma whipplei, molecular detection, PCR, varies-994

  
Tropheryma whipplei, molecular detection, PCR, varies
  
994
  
LAB994
  
MSO
  
Whipple's Disease
  

Aids in the diagnosis of Whipple disease, especially for identifying inconclusive or suspicious cases

  
Synovial fluid
  
Fluid: 0.5 mL
Fresh tissue or biopsy: 5 mm(3)
  
  • Container/tube:
    • Preferred: Lavender top (EDTA)
    • Acceptable: Navy (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container
  • Specimen volume: 0.5 mL
  • Send specimen in original tube (preferred)
  
  1. Place a minimum of 0.5 mL of fluid into a Lavender (EDTA) tube 
  2. Mix by gentle inversion
  
  
Fresh tissue or biopsy
Fluid
  

Fresh tissue or biopsy

  • Sources: Small intestine tissue (duodenum, ileum, or jejunum), lymph node, other visceral tissue, synovial tissue, gastrointestinal tissue, heart valve, or brain
  • Specimen volume: Entire collection or 5 mm3
    Collection instructions:

1. Collect fresh tissue specimen
2. Submit tissue only, do not add fluid to tissue
3. Refrigerate or freeze specimen

Fluid

  • Sources: Cerebrospinal or ocular (eg, vitreous humor)
  • Specimen volume: 0.5 mL

 

  

Sterile vial/container

  

Refrigerated (preferred): <7 days
Frozen: <7 days

 

 

  

PCR testing cannot be added on to a sample that has been opened and/or used for other testing. If the sample has already been used for testing, a new specimen will need to be collected.

  
Mayo Clinic Laboratories (TWRP): R-NX
  
Mo, We, Fr
  
2 - 7 days
  

Real-time Polymerase Chain Reaction (PCR)

  
1 week
  

Not applicable

  • A positive result indicates the presence of Tropheryma whipplei DNA.
  • A negative result indicates the absence of detectable T whipplei DNA, but does not negate the presence of the organism and may occur due to inhibition of PCR, sequence variability underlying primers or probes, or the presence of T whipplei DNA in quantities less than the limit of detection of the assay.
  
87798
  
No
  
11/06/2019
  
02/19/2020