T cell-restricted intracellular antigen by IHC

Alphabetical Test listing

T cell-restricted intracellular antigen by IHC-12376 - Technical only;12379 - Technical & interpretation

  
T cell-restricted intracellular antigen by IHC
  
12376 - Technical only
12379 - Technical & interpretation
  
LAB12376
LAB12379
  
TIA-1
  
  • All IHC stains will include a positive control tissue
  
  • TIA-1 is used (often in a panel with Granzyme B) to identify cytotoxic (NK-like) T-cell lymphomas, especially enteropathy associated T-cell lymphomas, and subcutaneous panniculitis-like T-cell lymphoma. These antibodies also identify true NK cell lymphomas/leukemias, including aggressive NK-cell leukemia/lymphoma and extra-nodal NK/T-cell lymphoma, nasal type
  • NK cell lymphomas are usually aggressive, and are associated with rapid progression, treatment failures, and relapses; therefore, accurate diagnosis is essential
  • Some studies report an associated of nasal-type NK cell lymphomas with EBV, and are more common in Asian and Latin American countries
  • P53 is also expressed in a high percentage of these types of lymphomas
  • TIA-1 should be used in a panel with other T cell markers, as well as with CD56 and Granzyme B (or perforin)
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • TIA-1 is a granule-associated cytotoxic protein, found in cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells
  • TIA-1 is expressed by NK cell lymphomas and by some T-cell lymphomas
  • TIA-1 expression is evidence of an activated state of CTL and NK cells, and is therefore useful in identifying related lymphomas associated with these types of cells

 Staining pattern

  • Granular cytoplasmic staining

References

  1. Gall K et al: Sinonasal NK/T-cell lymphomas in the United States. Am J Surg Pathol 24(11):1511-1517, 2000.
  2. Natkunam Y et al: Aggressive cutaneous NK and NK-like T-cell lymphomas; clinicopathologic, immunohistochemical and molecular analyses of 12 cases. Am J Surg Pathol 23(5): 571-581, 1999.
  3. Quintanilla-Martinez L et al: Histological and Immunophenotypic profile of nasal NK/T cell lymphomas from Peru: high prevalence of p53 over-expression. Hum Pathol 30:849-855, 1999.
  4. Kumar S et al: Subcutaneous panniculitic T-cell lymphoma is a tumor of cytotoxic T lymphocytes. Hum Pathol 29:397-403, 1998.
  
88342 - 1st stain
88341 - each additional stain
  
No
  
09/13/2017
  
10/23/2018
  
10/02/2020