B cell gene rearrangement

Alphabetical Test listing

B cell gene rearrangement-5453

Test name:

Test number:

5453

Excellian order number:

LAB5453

Abbreviation:

Alternate names:

IgH Rearrangement

Useful for:

Detection of clonal rearrangements of immunoglobulin heavy chain genes that are associated with malignant processes.

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL,

Volume:

4.0 mL

Minimum volume:

2.0 mL

Collection instructions:

Immediately after collection, mix tube thoroughly by gentle inversion, 8 - 10 times, to prevent clotting

Transport container:

Lavender (EDTA), 4mL,

Alternate specimen type:

4.0mL ACD whole blood
4.0mL Na Citrate whole blood
5.0cc bone marrow in EDTA
200mg FFPE tissue (B3/B5 not recommended)

Alternate collection container:

Yellow ACD (A or B)

Lt blue Sodium citrate (Na Cit) - 2.7mL

Bone Marrow

Lavender (EDTA), 4mL

Alternate specimen volume:

Whole blood 4.0mL (minimum 1.0 mL)
Bone marrow 5.0cc (minimum 1.0cc)
Tissue 200mg

Alternate processing instructions:

Whole blood/bone marrow

Submit entire specimen

Tissue

Frozen preferred if not tested immediately
Freeze as quickly as possible at -20 to -70°C
Fresh tissues may be submitted in RPMI
Best results when used within 3 days
Recommended for tissues with high probability of immediate or pending orders
Formalin fixed tissue in a paraffin block (B3 and B5 fixatives are not recommended)

Questions call (612) 863-4475

Alternate transport container:

Yellow ACD (A or B)

Lt blue Sodium citrate (NaCit)

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

Formalin-fixed, paraffin embedded (FFPE) tissue block

Required forms/information:

Molecular Medicare billing request

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
All information requested is required in order for your request to be completed

Transport and stability:

Whole blood or bone marrow

Refrigerated (preferred)

Ambient (OK)

Tissue

Ambient

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper blood/anticoagulant ratio
Delay in transport
Improper storage
Inappropriate timing of collection
Interfering substances
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Molecular Diagnostics: D

Days set up:

Varies

TAT:

8 days

Method:

PCR and Capillary electrophoresis

Preauthorization:

This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.

CPT codes:

81261

Subject to medical necessity (Y/N):

Yes

Other billing information:

Medical necessity

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
All information requested is required in order for your request to be completed

Molecular Medicare billing request

Date created:

06/22/2006

Revised date:

03/09/2022

Review date:

01/15/2024