The ELISA procedure utilizes the excretory-secretory(es) antigen of T. canis larvae to minimize cross-reactivity with antigens of Ascaris spp. and of other parasites. Results must be interpreted with caution as broad variations in antibody response occur and levels may remain elevated for years after infection. Generally, antibody levels are highest in visceral larva migrans (VLM). A negative result usually rules out infection with Toxocara spp.
Immediatley following collection, mix sample by gently inverting 5 times
Gold serum separator (SST) tube
Refrigerated (preferred) - 14 days
Ambient - 7 days
Frozen - 30 days
Enzyme-linked immunosorbent assay (ELISA)
Negative