Calreticulin exon 9 assay

Alphabetical Test listing

Calreticulin exon 9 assay-12372

Test name:

Test number:

12372

Excellian order number:

LAB12372

Abbreviation:

CALR

Useful for:

The Calreticulin Exon 9 assay detects insertions or deletions that result in frameshift mutations, which alter the resulting protein's function

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

3.0 mL

Collection instructions:

Only one (1) tube needs to be drawn for any combination of the following tests:
- Calreticulin exon 9 assay
- Factor V leiden mutation
- Factor 2 mutation
- Hereditary hemochromatosis
- JAK2 V617F mutation detection
- MTHFR C677T
Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

Transport container:

Lavender (EDTA), 4mL

Alternate specimen type:

Sodium citrate (Na cit) whole blood
ACD whole blood
Bone marrow

Alternate collection container:

Whole blood:

Lt blue Sodium citrate (Na Cit) - 2.7mL

Yellow ACD (A or B)

Alternate transport container:

Whole blood:

Lt blue Sodium citrate (NaCit)

Yellow ACD (A or B)

Bone marrow:

Lavender (EDTA), 4mL

Required forms/information:

Molecular Medicare billing request

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
All information requested is required in order for your request to be completed

Transport and stability:

Ambient (preferred)

Refrigerated (acceptable)

Reason for rejection:

Improper label (unlabeled or mislabeled)
Wrong container (anticoagulant or solution)
Improper blood/anticoagulant ratio
Delay in transport
Improper storage
Inappropriate timing of collection
Interfering substances
Leaking container
Slides broken beyond repair

Performing lab:

AHL - Molecular Diagnostics: D

Days set up:

Varies

TAT:

8 days

Method:

Polymerase Chain Reaction (PCR) + capillary electrophoresis

Reference ranges:

No mutation detected

Clinical information:

These CALR mutations are seen in 67% to 88% of patients with essential thrombocythemia or primary myelofibrosis who lack JAK2 or MPL mutations (JAK2, MPL, and CALR mutations are mutually exclusive). CALR mutations are not limited to patients with myeloproliferative neoplasms as they can be seen infrequently in patients with myelodysplastic syndromes or mixed myelodysplastic/myeloproliferative syndromes. The Calreticulin Exon 9 Assay can be ordered as a reflex to a negative JAK2 V617F Mutation Detection. The reflex must be indicated on the request form or in the Excellian order. If ordered as a reflex, do not order the individual assay.

Preauthorization:

This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.

CPT codes:

81219

Subject to medical necessity (Y/N):

Yes

Other billing information:

Medical necessity

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medical billing request form along with the sample.

Complete and submit the form to notify us of the need for Allina Health Laboratory to bill insurance for Molecular testing performed
All information requested is required in order for your request to be completed

Molecular Medicare billing request

Date created:

09/22/2014

Revised date:

07/27/2018

Review date:

01/15/2024