CD35 by IHC

Alphabetical Test listing

CD35 by IHC-12376 - Technical only, 12379 - Technical & interpretation

Test name:

CD35 by IHC

Test number:

12376 - Technical only, 12379 - Technical & interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

Anti-human dendritic reticulum cell

Tests included:

All IHC stains will include a positive control tissue

Useful for:

CD35 is useful in identifying the follicular dendritic cells in non-Hodgkin's lymphomas, seen as a sharply defined area of nodular staining
This antibody can also show a loose, ill-defined area of staining in some diffuse lymphomas
Precursor B NHL such as lymphoblastic lymphomas, and Burkitt lymphomas, plasmocytomas, and hairy cell leukemias show no staining for CD35
Nodular lymphocyte predominant Hodgkin's disease will show CD35 staining in a follicular pattern; all other types of HD are negative
CD 35 is most useful in a panel with CD21 and CD23 to identify follicular dendritic cell sarcoma/tumor

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin-embedded tissue

Transport container:

Formalin-fixed, paraffin-embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

CD35 antibody reacts with a receptor of the C3b fragment of compliment (To5)
CD35 labels follicular dendritic cells in lymphoid follicles, and to a lesser degree, mantle zone lymphocytes
CD35 stains the C3b receptor on epithelial cells of renal glomeruli
CD35 will also stain macrophages and histiocytes

Staining pattern

Membrane -based staining

References

Chan JKC: Proliferative lesions of follicular dendritic cells: an overview, including a detailed account of follicular dendritic cell sarcoma, a neoplasm with many faces and uncommon etiologic associations. Adv Anat Pathol, 1997; 4(6); 347-411.
Nguyen DT et al: Follicular dendritic cell sacrum. Identification by monoclonal antibodies in paraffin sections. Appl Immunohistochem 2:60-64.

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

06/16/2017

Revised date:

01/31/2024

Review date:

01/31/2022