Terminal deoxynucleotidyl transferase by IHC

Alphabetical Test listing

Terminal deoxynucleotidyl transferase by IHC-12376 - Technical only;12379 - Technical & interpretation

  
Terminal deoxynucleotidyl transferase by IHC
  
12376 - Technical only
12379 - Technical & interpretation
  
LAB12376
LAB12379
  
Tdt
  
  • All IHC stains will include a positive control tissue
  
  • Tdt identifies immature T and B lymphocyte precursors in acute leukemias. It is expressed at a high level in T cell and pre-B cell ALL and lymphoblastic lymphomas
  • Tdt is very useful on bone marrow trephines of leukemias where an adequate aspirate was unable to be obtained
  • B cell ALL (Burkitt type) and mature B and T cell malignancies are Tdt negative
  • Tdt may be expressed in some cases of AML and CML (blast crisis)
  • Tdt Expression is normally seen in the thymus (as well as in thymomas) and in a few cells in normal bone marrow (corresponding to normal precursor cells)
  
Tissue
  

Submit a formalin-fixed, paraffin embedded tissue block

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

FFPE tissue section mounted on a charged, unstained slide

  

Ambient (preferred)

  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining and microscopic examination

  

If requested, an interpretive report will be provided

  

Specifications

  • Tdt recognizes a DNA polymerase that is present in the nuclei of normal and neoplastic T and B lymphocyte precursors

 Staining pattern

  • Nuclear based staining

References

  1. Orazi et. al.: TdT Staining
  
88342 - 1st stain
88341 - each additional stain
  
No
  
09/13/2017
  
10/17/2018