Gold serum separator (SST) tube

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
   Spin within two (2) hours of sample collection
2. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum and as acute or convalescent serum

Transfer vial/tube with cap - 12mL (LabCorp)

Red serum vial/tube - 5 mL

1. Allow sample to clot 
2. Spin within two (2) hours of sample collection
3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum and as acute or convalescent serum

Refrigerated (preferred) - 9 months

Ambient - 2 weeks

Frozen - 1 year

• Gross hemolysis
• Gross lipemia
• Specimen contaminated with bacteria and/or fungus
• Serum sample containing an additive detrimental to the assay (i.e. EDTA)

Rapid Fluorescent Focus Inhibition Test (RFFIT)

0.1 to 15.0IU/mL

Results are reported as either <0.1 IU/mL, ≥ 0.1 IU/mL or ≥ 0.5 IU/mL.

RFFIT stands for Rapid Fluorescent Foci Inhibition Test. It is a serum neutralization (inhibition) test, which means it measures the ability of rabies specific antibodies to neutralize rabies virus and prevent the virus from infecting cells. These antibodies are called rabies virus neutralizing antibodies (RVNA).