Luteinizing hormone by IHC

Alphabetical Test listing

Luteinizing hormone by IHC-12376 - Technical only, 12379 - Technical & Interpretation

Test name:

Test number:

12376 - Technical only, 12379 - Technical & Interpretation

Excellian order number:

LAB12376
LAB12379

Alternate names:

Tests included:

All IHC stains will include a positive control tissue

Useful for:

Immunoperoxidase staining for the presence of pituitary hormones helps distinguish pituitary adenomas and the rare primary pituitary carcinomas from other tumors occurring in the region of the sella (such as craniopharyngioma, metastatic carcinoma, meningioma, lymphoma, leukemia and plasmacytoma), none of which contain or produce pituitary hormones. Positive staining for specific pituitary hormones can divide the pituitary adenomas into subclasses based on the corresponding cell type present
Negative staining for pituitary hormones does not rule out a pituitary adenoma since some contain none of the known adenohypophyseal hormones
Gonadotrophic cell adenomas account for 10-15% of all pituitary adenomas. They usually stain for both LH and FSH

Specimen type:

Tissue

Processing instructions:

Submit a formalin-fixed, paraffin embedded (FFPE) tissue block

Transport container:

Formalin-fixed, paraffin embedded (FFPE) tissue block

Alternate transport container:

FFPE tissue section mounted on a charged, unstained slide

Transport and stability:

Ambient (preferred)

Reason for rejection:

Unlabeled/mislabeled block
Insufficient tissue
Slides broken beyond repair

Performing lab:

AHL - Immunohistochemistry

Days set up:

Mo - Fr

TAT:

1 - 2 days

Method:

Immunohistochemical staining and microscopic examination

Reference ranges:

If requested, an interpretive report will be provided

Clinical information:

Specifications

This antibody reacts with LH as well as the separate alpha and beta LH subunits. It shows 8.1% cross-reactivity with TSH and 11.3% cross-reactivity with HCG (alpha subunit). It recognizes the gonadotropic cells of the pituitary gland. In the normal pituitary gland, the gonadotrophs comprise about 10% of the cells and are scattered throughout the adenohypophysis with some concentration in the posterolateral region. In some gonadotrophs, FSH and LH can be demonstrated in the same cell, but most gonadotrophs contain only one of the hormones

Staining pattern

Cytoplasmic based staining

LH IHC

CPT codes:

88342 - 1st stain
88341 - each additional stain

Date created:

07/16/2017

Revised date:

01/31/2024

Review date:

01/31/2022