Direct antiglobulin test

Alphabetical Test listing

Direct antiglobulin test-6085

Test name:

Test number:

6085

Excellian order number:

86880.3

Abbreviation:

DAT

Alternate names:

Coombs, direct
DAT

Tests included:

Extended DATs using IgG and C-3 specific antisera may be performed at additional charge if Coombs, Direct is positive
- An RBC elution and any necessary antibody ID may be performed at additional charge if indicated

Useful for:

Demonstrating in vivo coating of red blood cells with IgG or the complement component C3d in the following settings:

Autoimmune heomlytic anemia
Hemolytic or delayed transfusion reactions
Drug induced hemolytic anemia

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

4.0 mL

Collection instructions:

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Labelling instructions:

Excellian sites:
Tube must be labeled with the patients first and last name and Excellian MRN, date and time of collection and phlebotomist’s A#

Non-Excellian sites:
Tube must be labeled with the patient's first and last name and date of birth, date and time of collection and phlebotomist’s initials.

Transport container:

Lavender (EDTA), 4mL

Transport and stability:

Refrigerate - 3 days

Sample should be obtained no more than 24 hours prior to testing but may be used up to 3 days post collection. Testing of older specimens may show decreased antibody reactivity.

Reason for rejection:

Improper label (unlabeled or mislabeled)
Hemolysis

Performing lab:

AHL - Blood Bank: B

Days set up:

Daily

TAT:

1 day

Method:

Column-agglutination, agglutination

Reference ranges:

Negative

Clinical information:

The direct antiglobulin test (DAT) is a screening test to determine if red cells have been coated in vivo with immunoglobulin, complement, or both.

CPT codes:

86880

Date created:

11/30/2001

Revised date:

12/27/2023

Review date:

12/21/2023