PDGFRA (CHIC2) by FISH

Alphabetical Test listing

PDGFRA (CHIC2) by FISH-4280G (bone marrow) or 4280H (blood)

  
PDGFRA (CHIC2) by FISH
  
4280G (bone marrow) or 4280H (blood)
  
4280G - CYTOGENETIC BONE MARROW STUDIES
4280H - CYTOGENETIC LEUKEMIC BLOOD STUDIES
  
PDGFRA (CHIC2) FISH
  
CHIC2
Eosinophilia
Eosinophilia FISH Panel
FIP1L1
FIP1L1-PDGFRA fusion
Mast Cell Disease
PDGFRA
4q12
Fluorescence In Situ Hybridization
FISH
  

Genetic abnormalities that involve the PDGFRA gene cause a type of blood cell cancer called PDGFRA-associated chronic eosinophilic leukemia. The most common of these genetic abnormalities is a deletion of genetic material from chromosome 4 that brings together parts of two genes, FIP1L1 and PDGFRA, creating the FIP1L1-PDGFRA fusion gene. Occasionally, genes other than FIP1L1 are fused with the PDGFRA gene via a translocation between chromosome 4q12 and a partner chromosome. This gene is also involved in systemic mastocytosis and chronic eosinophilic leukemia.  Patients carrying the FIP1L1-PDGFRA fusion show an excellent response to low-dose imatinib mesylate therapy.

This test can be ordered individually or as part of an Eosinophilia FISH Panel. See the individual panel test for more details.

  
Bone Marrow aspirate
  

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

  

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel

 

  
5 mL
  
3 mL
  

Transfer aspirate from syringe to transport container. 

  

Submit entire specimen unspun

  

Sterile vial/tube

  

or

Dk green Sodium heparin (Na hep), no gel

  
Bone marrow core
Sodium heparinized whole blood
Touch Prep Slides
  

Bone marrow core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Whole blood:

Dk green Sodium heparin (Na hep), no gel

  
Bone marrow core: 10 mm
Whole blood: 5 mL
  

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting.

  

Submit entire specimen unspun

  

Bone marrow core:

Sterile tube/cup

Whole blood:

Dk green Sodium heparin (Na hep), no gel

  

AHL Cytogenetics Oncology Request

  

Ambient

  
Specimens retained until completion of all testing
  
  • Improper label (unlabeled or mislabeled)
  • Wrong container (anticoagulant or solution)
  • Delay in transport
  • Improper storage
  • Leaking container
  • Slides broken beyond repair
  
AHL - Cytogenetics: X
  
Mo-Fr
  
14 business days
  

Fluorescence in situ hybridization (FISH)

  

Normal or Abnormal with interpretation

  
88271 x 3
88275
88291
  
Yes
  
  
03/16/2021
  
09/08/2022
  
09/08/2022