Quantitative Lymphocyte Subsets: T, B, and Natural Killer panel

Alphabetical Test listing

Quantitative Lymphocyte Subsets: T, B, and Natural Killer panel-14271

Test name:

Test number:

14271

Excellian order number:

LAB14271

Abbreviation:

TBNK

Alternate names:

CD4/CD8 ratio
Helper/Suppressor ratio
IDP
Immunodeficiency panel
Lymphocyte subsets
T cell subsets
T, B, and/or NK enumeration
T&B subsets

Tests included:

Percentage and absolute counts for:

Total T-cells (CD3+)
B-cells (CD3-/CD19+)
Natural killer (NK) cells (CD3-/CD56+CD16+)
Helper/inducer (CD3+/CD4+) T-cell subset
cytotoxic/suppressor (CD3+/CD8+) T-cell subset
Total CD45+ lymphocyte count
CD4:CD8 ratio

No pathologist morphology or interpretation is provided

Useful for:

Immunological assessment of normal individuals and patients having, or suspected of having, immunodeficiency
Initial screening and follow up monitoring of patients with primary immunodeficiencies
Assessment of immune reconstitution post hematopoietic cell transplantation
Screening for gross quantitative anomalies in lymphocyte subsets in infection or malignancies
Any clinical scenario where enumeration of major lymphocyte subsets in peripheral blood is indicated

Limitations:

This test is not intended for the detection of phenotypically aberrant lymphocytes or blasts; for the detection of aberrant lymphocytes or blasts, a peripheral blood morphology should be ordered

Specimen type:

EDTA whole blood

Collection container:

Lavender (EDTA), 4mL

Volume:

4.0 mL

Minimum volume:

1.0 mL

Collection instructions:

Immediately following collection, mix tube thoroughly by gentle inversion, 8 - 10 times, to prevent clotting

Processing instructions:

Submit whole blood specimen in lavender (EDTA) tube
Do not spin

Transport container:

Whole blood:

Lavender (EDTA), 4mL

Transport and stability:

Ambient (strict) - 72 hours

Due to gradual cell degradation over time, testing within 24 hours is strongly recommended. However, specimens up to 72 hours will be accepted.

Refrigerated - NO

Frozen - NO

Reason for rejection:

Wrong container (anticoagulant or solution)
Improper blood/anticoagulant ratio
Delay in transport
Improper storage
Interfering substances
Leaking container
Cellular viability less than 50%
Improper label (unlabeled or mislabeled)

Performing lab:

AHL - Flow Cytometry: F

Days set up:

Mo - Fr

TAT:

2 days

Method:

BD Multitest 6-Color TBNK reagent with Trucount tubes

Reference ranges:

Appropriate age-specific reference values will be provided on the report

CPT codes:

86355
86357
86359
86360

LOINC code:

80721-4

Date created:

02/25/2020

Revised date:

08/12/2022

Review date:

01/09/2024