Inhibin by IHC

Alphabetical Test listing

Inhibin by IHC-12376 - Technical only; 12379 - Technical & Interpretation

  
Inhibin by IHC
  
12376 - Technical only
12379 - Technical & Interpretation
  
LAB12376
LAB12379
  
IHC
  
  • Immunohistochemical staining with antibodies to inhibin is of value in the diagnosis of granulosa cell and other sex cord - stromal tumors and in the distinction of these neoplasms from mimics
  • 90-100% of adult and juvenile granulosa cell tumors and other sex cord stromal proliferations (including steroid cell tumors) show moderate to strong intensity staining in at least half the cells
  • 70-80% of ovarian fibro-thecomas are positive for inhibin
  • 10-15% of:
    • primary ovarian endometrioid tumors
    • primary ovarian transitional cell (Banner) proliferations
    • Other primary and metastatic poorly differentiated (undifferentiated) carcinomas can be positive for inhibin but usually show weak staining or staining in less than half of the cells
    • Germ cell neoplasms are negative for inhibin
    • Caution should be exercised in the interpretation of inhibin positive cells because a wide variety of primary and metastatic ovarian tumors may contain significant numbers of positively staining luteinized cells
    • Tissue staining of inhibin does not correlate with serum levels of inhibin
    • Inhibin staining is present in normal adrenal gland, adrenocortical adenomas and adrenocortical carcinomas. Paragangliomas* and renal cell carcinoma

are negative for inhibin. (*Rare cases may show focal positive)

References:

  1. Hildebrandt RH, et. al.: Value of Inhibin in the Identification of Granulosa Cell Tumors of the Ovary. Human Pathol 28:1387-1395, 1997.
  2. McCaveeage WG, et. al.: Immunohistochemical Staining of Ovarian Granulosa Cell Tumors with Monoclonal Antibody Against Inhibin. Hum Pathol 28:1034-1038, 1997.
  3. Costa MJ et. al.: Inhibin Immunohistochemistry Applied to Ovarian Neoplasm: A Novel Effective Diagnostic Tool. Hum Pathol 28:1247-1254, 1997.
  
Tissue
  

Submit processed tissue block or tissue section mounted on a charged, unstained slide

  

Formalin-fixed, paraffin embedded (FFPE) tissue block

  

Ambient (preferred)

  
AHL - Immunohistochemistry
  
Mo - Fr
  
1 - 2 days
  

Immunohistochemical staining

Microscopic examination

  

If requested, an interpretive report will be provided

  

All IHC stains will include a positive control tissue

Specifications:

  • Antibody recognizes 32kD alpha subunit of human inhibin
  • Inhibins are peptide hormones that help regulate the pituitary-gonadal feedback system
  • Inhibins are selectively expressed by cells of sex cord - stromal derivation

 
Staining pattern:

  • Cytoplasmic
  
88342 - 1st stain
88341 - each additional stain
  
07/16/2017
  
10/19/2018