Detection of carbapenem resistant organisms (CROs) in human rectal swab specimens.

Mutations or polymorphisms in primer or probe binding regions may affect detection of current, new or unknown KPC, NDM, VIM, OXA-48, and IMP variants, resulting in a false negative result.

As the detection of KPC, NDM, VIM, OXA-48, and IMP gene sequences is dependent on the number of organisms present in the sample, reliable results are dependent on proper sample handling and storage.

This order is for Allina Health only.  Non-Allina sites need to contact MDH directly.

Dual Swab in Liquid Stuart Media (Red cap dual collection bacterial swab)

1. Pull the dual swab from the plastic packaging, being careful not to touch the cotton tips.
2. Carefully insert both swab tips approximately 1 cm beyond the anal sphincter and gently rotate against the walls of the rectum (3) three times

Diapered infants: The cotton swab may be used to swab the stool present in the soiled diaper.

Patients with an ostomy: Use the cotton applicator to obtain specimen from the stoma site.

      3. Confirm swab is not overloaded or underloaded.                   

      4. Insert dual swab into tube and firmly close cap.

Dual Swab in Liquid Stuart Media (Red cap dual collection bacterial swab)

Ambient - 5 days

• Specimen >5 days old.
• Swabs other than Dual Swab in Liquid Stuart Media
• Swabs heavily soiled with stool.

Real-Time Polymerase Chain Reaction (RT-PCR)

IMP (Not Detected)

VIM (Not Detected)

NDM (Not Detected)

KPC (Not Detected)

OXA (Not Detected)