All IHC stains will include a positive control tissue

• Approximately 90% of peripheral T cell lymphomas are positive for Leu22
• However, its disadvantage is that 30-40% of B cell NHL are also Leu22 positive
• Approximately 90% of myeloid leukemias are positive for Leu22
• The combination of CD20 positive and Leu22 positive lymphoid populations is diagnostic of malignant lymphoma

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• CD43 reacts with a membrane/cytoplasmic antigen expressed by T cells and myeloid lineage cells

Staining pattern

• Cell membrane based

References

1. Ngan et. al.: Immunophenotypic Diagnosis of Non-Hodgkin's Lymphoma in Paraffin Sections: Co-expression of Leu22 and L26 Antigens Correlates with Malignant Histology; American Journal of Clinical Pathology 91:579-583, 1989.