• All IHC stains will include a positive control tissue

• KP1 reacts with cells of monocyte/macrophage or myelomonocytic lineage (this includes epithelial histiocytes, multinucleated histiocytes, osteoclasts, giant cells in giant cell tumors of bone, Gaucher's cells, sea blue histiocytes)
• KP1 will also react with neutrophils, mast cells and basophils; and will stain myeloid leukemias (useful in the workup of a chloroma)
• KP1 can stain a wide variety of non-Hodgkin's lymphomas of B-cell phenotype (most frequently KP1 positive NHL's are small lymphocytic and diffuse, small cleaved NHL)
• KP1 stains granular cell tumors, and Schwann cells in benign peripheral nerve sheath tumors
• KP1 is not highly specific; KP1 also may react with hepatocytes, kidney tubules and glomeruli, pancreatic acini and islets, skin epithelium, sweat glands, connective tissue, and endothelial cells
• KP1 reactivity has also been reported in MFH, renal cell carcinoma, malignant melanoma, meningioma, glioblastoma, infiltrating duct carcinoma of the breast

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• CD68/KP1 is an antibody that recognizes an intracellular glycoprotein associated with lysosomes, phagosomes, and primary granules of neutrophils. It is a very useful marker for identifying macrophages/histiocytes

Staining pattern

• Granular cytoplasmic based staining

References

1. Weiss, et. al.: CD68; A Review; Appl. Immunohistochemistry 2(1):2-8, 1994.
2. Horny, et. al.: Assessment of the Value of Immunohistochemistry in the Subtyping of Acute Leukemia on Routinely Processed Bone Marrow Biopsy.