This test useful for the evaluation of possible hemolysis including clotting/hemolysis associated with left ventricular assist devices. Measurement of free Hgb in plasma is of no practical value in the diagnosis of chronic hemolytic anemias.

Lt green plasma separator (PST)

• Use no smaller than a 21 gauge needle for specimen collection
• Immediately following collection, mix sample thoroughly by gentle inverting 8 - 10 times to prevent clotting

1. Spin immediately
2. Transfer plasma to a False bottom plasma/serum transport vial/tube (AHL) 
3. Spin a second time
4. Transfer plasma to a new False bottom plasma/serum transport vial/tube (AHL), labelled as Li Hep plasma

False bottom plasma/serum transport vial/tube (AHL)

Gold serum separator (SST) tube

Red serum vial/tube, 5 mL

Gold:

Immediately following collection, thoroughly mix sample by gently inverting 5 times

Gold:

1. Allow sample to clot for a minimum of 30 minutes
2. Spin immediately
3. Transfer serum to a False bottom plasma/serum transport vial/tube (AHL) 
4. Spin a second time
5. Transfer serum to a new False bottom plasma/serum transport vial/tube (AHL), labelled as serum plasma

Red:

1. Allow sample to clot
2. Spin immediately
3. Transfer serum to a False bottom plasma/serum transport vial/tube (AHL) 
4. Spin a second time
5. Transfer serum to a new False bottom plasma/serum transport vial/tube (AHL), labelled as serum plasma

Refrigerated

• Improper labels (unlabeled or mislabeled)
• Hemolysis (some procedures)
• Improper anticoagulant or ratio
• Delay in transport
• Improper storage temperature affecting results
• Improper container
• Improper processing
• Leaking container resulting in compromised specimen
• Quantity not sufficient (QNS)

HemoCue low plasma hemoglobin

< 40 mg/dL