Serum methylmalonic acid (MMA) measurement is used to evaluate individuals with signs and symptoms associated with vitamin B12 deficiency or congenital methylmalonic academia.

Gold serum separator (SST) tube

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within one (1) hour of sample collection

Gold serum separator (SST) tube

Red serum vial/tube, 5 mL

Dk green heparin (Li or Na), no gel

Dk green:

Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting

Red:

1. Allow sample to clot
2. Spin within one (1) hours of sample collection
3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum

Dk green:

1. Spin within one (1) hours of sample collection
2. Transfer plasma to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as the appropriate plasma type

Transfer vial/tube with cap - 12mL (LabCorp)

Refrigerated (preferred) - 14 days

Ambient - 14 days

Frozen - 19 days

Freeze/thaw cycles - stable x 3

• Plasma from a light blue-top (citrate)
• Plasma from a yellow-top tube (ACD) 

Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

0 - 378 nmol/L