• Testosterone, Total, LC/MS
• Testosterone, Free
• % Free Testosterone

• Evaluate hirsutism and masculinization in women
• Evaluate testicular function in clinical states in which the testosterone-binding proteins may be altered (obesity, cirrhosis, thyroid disorders)

Gold serum separator (SST) tube x 2

Note: Collect 2 Gold SST tubes

Immediately following collection, mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin and transfer aliquot within one (1) hour of sample collection

Gold serum separator (SST) tube

Red serum vial/tube, 10 mL

Red serum vial/tube, 5 mL

1. Allow sample to clot
2. Spin within one (1) hour of sample collection
3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum

Transfer vial/tube with cap - 12mL (LabCorp)

Ambient (preferred)- 7 days

Refrigerated - 7 days

Frozen - 14 days

Freeze/thaw cycles - stable x9

• Hemolyzed or icteric sample
• Gross lipemia (may yield erroneously high testosterone results)
• Recently administered isotopes
• Plasma specimen received
• Improper labeling
• Specimen drawn in citrate or ACD tube

Free: Equilibrium ultrafiltration

Total: Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

See report