All IHC stains will include a positive control tissue

• CD57 stains the small lymphocytes in nodular lymphocyte predominance Hodgkin's disease (NLPHD), in a ring-like pattern surrounding L&H cells (this pattern can distinguish NLPHD from nodular sclerosing Hodgkin's disease, T-cell-rich B-cell lymphoma, and follicular NHL
• CD57 is useful in tumor diagnosis as a specific but relatively insensitive pan-neuroendocrine marker, usually showing positive staining in neuroblastomas, retinoblastomas, PNETs, neuroepitheliomas, carcinoids, Merkel cell tumors of the skin, paragangliomas, pheochromocytomas, small cell neuroendocrine carcinomas of the lung, urinary bladder, colon, rectum, and endometrium, medullary carcinomas of the thyroid, and some pancreatic endocrine tumors
• Occasional cases of Ewing's sarcoma, granular cell myoblastoma, Wilms' tumor, leiomyosarcoma, synovial sarcoma, epithelioid sarcoma, clear cell sarcoma, embryonal rhabdomyosarcomas, and malignant melanomas also stain positively for CD57
• Positive staining is also seen in other neural tumors such as schwannomas, neurofibromas, neurofibrosarcomas, astrocytomas and ependymomas
• A high percentage of oligodendrogliomas show positive cell surface membrane staining for CD57
• Positive staining for CD57 is seen in normal and hyperplastic prostate and prostatic adenocarcinomas
• CD57 is absent in squamous cell carcinomas and adenocarcinomas (other thanreports of rare prostate tumors as above)

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Anti CD57 recognizes a 110 KD glycoprotein human lymphocyte antigen that is present on approx. 25% of adult peripheral blood mononuclear cells
• CD57 is expressed on a subset of NK cells and a subset of germinal center T-cells
• This antibody also recognizes a carbohydrate epitope on sub-populations of several cell adhesion molecules including N-CAM and myelin-associated glycoprotein (MAG)
• In normal tissues, CD57 stains a common neuro-endodermal antigen with positivity in cells of the CNS, the adrenal medulla, islets of Langerhans and the cells of the APUD system in the lung and gut

 Staining pattern

• Cytoplasmic and cell surface membrane based staining

References

1. Kamel OW, et al: Leu 7 (CD57) reactivity distinguishes nodular lymphocyte predominance Hodgkin's disease from nodular sclerosing Hodgkin's disease, T-cell- rich B-cell lymphoma and follicular lymphoma. American Journal of Pathology, Vol 142, 541-546, 1993.
2. Arber DA, Weiss LM: CD57: a review. Appl Immuno 1995, 3:137-152.
3. Michels S et al: Leu-7 in small cell neoplasms. An immunohistochemical study with ultrastructural correlations. Cancer 1987, 60:2958-2964.