MTHFR C677T assay-5919
Restricted to Allina Health and Affiliate provider orders only.
Test info
Specimen
- Only one (1) tube needs to be drawn for any combination of the following tests:
- Calreticulin Exon 9 Assay
- Factor V Leiden Mutation
- Factor 2 Mutation
- Hereditary Hemochromatosis
- JAK2 V617F Mutation Detection
- MTHFR C677T assay
- Immediately following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting
Lavender (EDTA), 4mL
Sodium citrate (Na cit) whole blood
Lt blue Sodium citrate (NaCit)
Yellow ACD (A or B)
Molecular Medicare billing request
Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medicare billing request form to notify us of the need for Allina Health Laboratory to bill insurance.
Ambient (preferred)
Refrigerated
- Improper label (unlabeled or mislabeled)
- Wrong container (anticoagulant or solution)
- Improper blood/anticoagulant ratio
- Delay in transport
- Improper storage
- Interfering substances
Performance
Polymerase Chain Reaction (PCR) with RFLP analysis
Clinical and Interpretive info
Negative
Due to lack of established clinical utility, MTHFR genotyping is no longer covered by most insurances. MTHFR genotyping should not be ordered as a part of the clinical evaluation for thrombophilia or recurrent pregnancy loss; fasting homocysteine is the preferred biomarker in these cases. The use of MTHFR genotyping should be restricted to symptomatic patients who have an increased basal homocysteine. The lack of evidence to support MTHFR variant testing is detailed in a 2013 practice guideline from the American College of Medical Genetics and Genomics (ACMG). MTHFR genotyping is considered investigational for nutritional, mental health or pharmacogenic assessment. The indication for testing must be provided at the time of order. This is a genetic test and needs to be performed only once in a lifetime
Billing
Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medicare billing request form to notify us of the need for Allina Health Laboratory to bill insurance.