• All IHC stains will include a positive control tissue

• T Pallidum is used to identify syphilis in tissue based material
• This stain is an important adjunct in the identification of syphilis, since the organism is refractory to culture, and histochemical stains are difficult to interpret

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• T Pallidum identifies the causative agent of syphilis

 Staining pattern

• Red, corkscrew bacterial pattern of staining (a red chromogen is used with this antibody)

References

1. Guarner J et al: Congenital syphilis in a newborn: an immunopathologic study. Mod Pathol 1999; 12:82-87.
2. Beckett JH et al: Immunoperoxidase localization of treponema pallidum: its use in formaldehyde-fixed and paraffin-embedded tissue sections. Arch Pathol Lab med 1979 Mar; 103(3): 135-8.