• All IHC stains will include a positive control tissue

• Immunoperoxidase staining for the presence of pituitary hormones helps distinguish pituitary adenomas and the rare primary pituitary carcinomas from other tumors occurring in the region of the sella (such as craniopharyngioma, metastatic carcinoma, meningioma, lymphoma, leukemia, and plasmacytoma), none of which contain or produce pituitary hormones. Positive staining for specific pituitary hormones can divide the pituitary adenomas into subclasses based on the corresponding cell type present
• Negative staining for pituitary hormones does not rule out a pituitary adenoma since some contain none of the known adenohypophyseal hormones.
• Thyrotrophic cell adenomas are the least common form of pituitary adenoma (1% of total)
• TSH expression is common (with GH and PRL) in Pluri-hormonal adenomas and in some cases the TSH reaction predominate

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• TSH recognizes the thyrotrophic cells of the pituitary gland. In the normal pituitary gland, these are mostly contained in the central portion of the adenohypophysis (anteromedial segments) and account for approximately 5% of the cells in the gland. This antibody also shows 4% cross-reactivity for LH, 1% for FSH, 1.5% for HCG, and less than 1% for hGH

 Staining pattern

• Cytoplasmic based staining