MLL (CDKN2A) or t(11q23;?) rearrangements are recurring abnormalities in AML, B-cell ALL and MDS. According to current NCCN AML guidelines, these abnormalities are classified as adverse cytogenetic risk factors, however, a t(9;11) is classified as an intermediate risk factor.  All MLL rearrangements are classified as intermediate cytogenetic risk factors in MDS. 

This test can be ordered individually or as part of the B-ALL or MDS FISH Panels. See the panel tests for more details.

Bone marrow aspirate:

Collect bone marrow aspirate in a sterile Sodium heparin (Na hep) syringe and transfer to a sterile vial/tube

- or -

Collect bone marrow in sterile syringe (non-heparinized) and transfer to a Dk green Sodium heparin (Na hep), no gel

Transfer aspirate from syringe to transport container. 

Submit entire specimen unspun.

Sterile vial/tube

or

Dk green Sodium heparin (Na hep), no gel - 4mL

Bone marrow core:

Submit in sterile tube/cup filled with sterile culture medium, sterile Hanks Balance Salt Solution (HBSS) or sterile saline

Whole blood:

Dk green Sodium heparin (Na hep), no gel - 4mL

Bone marrow core:

Sterile tube or cup

or 

Whole blood:

Dk green Sodium heparin (Na hep), no gel

Submit entire specimen unspun

Bone marrow core:

Sterile tube/cup

Whole blood:

Dk green Sodium heparin (Na hep), no gel

AHL Cytogenetics Oncology Requisition

Ambient

• Improper label (unlabeled or mislabeled)
• Wrong container (anticoagulant or solution)
• Delay in transport
• Improper storage
• Leaking container
• Slides broken beyond repair

Fluorescence in situ hybridization (FISH)

Normal or Abnormal with interpretation

Medical necessity