SeroNeg Rad x 3 profile-994
Test info
- 14-3-3 eta protein
- Anti-CEP-1 Ab
- Anti-Sa Ab
SeroNeg RAdx3 is a diagnostic and prognostic panel for Rheumatoid Arthritis designed to complement traditional RF and Anti-CCP testing.
This profile, consisting of 14-3-3 eta protein, Anti-CEP-1 Ab and Anti-Sa Ab, enhances the diagnosis of established or early RA (in individuals seronegative for RF and Anti-CCP Ab) and also helps predict disease severity.
Decrease in 14-3-3eta and/or Anti-Sa Ab with treatment is associated with less radiographic progression. The presence of Anti-CEP1 and Anti-CCP Antibodies suggests the imminent progression of pre-clinical RA into clinical RA.
Specimen
Immediately following collection, thoroughly mix sample by gently inverting 5 times
- Allow sample to clot for a minimum of 30 minutes
- Spin within one (1) hour of specimen collection
- Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum
Gold serum separator (SST) tube
- Allow sample to clot
- Spin within two (2) hours of sample collection
- Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum
Refrigerated (preferred) – 14 days
Ambient – 7 days
Frozen – 60 days
Freeze/thaw cycles - stable x1
- Grossly hemolyzed
- Bacterial contamination
- Lipemic specimen
- Icteric specimen
- Non-serum specimen types
Performance
Enzyme-linked Immunosorbent Assay (ELISA)
Clinical and Interpretive info
14.3.3 ETA, Rheum. Arthritis
Negative: <0.20 ng/mL
Weak positive: 0.20 - 0.39 ng/mL
Moderate positive: 0.40 - 0.79 ng/mL
Strong positive: >0.79 ng/mL
Anti-CEP-1 Ab, IgG; Units
Negative: <20
Weak positive: 20 - 39
Moderate positive: 40 - 80
Strong positive: >80
Anti-Sa Ab, IgG; Units
Negative: <20
Weak positive: 20 - 39
Moderate positive: 40 - 80
Strong positive: >80