• All IHC stains will include a positive control tissue

• HHV8 identifies a Herpes associated antigen found in Kaposi's sarcoma; HHV8 can be identified in the subcapsular spindle cell proliferations of lymph nodes, in early intranodal KS
• HHV8 is found in endothelial cells in Castleman's disease (in mantle zone large immunoblastic B cells)
• HHV8 is found in Primary effusion lymphomas (HIV related as well as in patients without HIV-induced immunodeficiency), and has also been reported in other HIV related lymphomas

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Recognizes a recombinant protein corresponding to a portion of the C-terminus of the latent nuclear antigen-1 molecule of HHV8
• HHV8 is used in the diagnosis of three malignancies, due to the association with HHV8 infection (casual agent): Kaposi's sarcoma (endemic, classic, and AIDS-related), primary effusion lymphoma, and some plasma cell forms of multicentric Castleman's disease

Staining pattern

• Nuclear, sometimes with a speckled pattern

References

1. Cheuk W et al: Immunostaining for human herpesvirus 8 latent nuclear antigen-1 helps distinguish Kaposi sarcoma from its mimickers. Am J Clin Pathol. 2004 Mar;121(3):335-42.
2. Patel RM et al: Immunohistochemical detection of human herpes virus-8 latent nuclear antigen-1 is useful in the diagnosis of Kaposi sarcoma. Mod Pathol. 2004 Apr;17(4):456-60.
3. Robin YM et al: Human herpesvirus 8 immunostaining: a sensitive and specific method for diagnosing Kaposi sarcoma in paraffin-embedded sections. Am J Clin Pathol. 2004 Mar;121(3):330-4.
4. Cioc AM et al: Oral plasmablastic lymphomas in AIDS patients are associated with human herpesvirus 8. Am J Surg Pathol. 2004 Jan;28(1):41-6.
5. Carbone A: AIDS-related NHL: from pathology and molecular pathogenesis to treatment. Hum Pathol 33:392-404.