Lt blue Sodium citrate (NaCit) - 2.7mL

• NaCitrate tubes must be filled to provide proper blood to anticoagulant ratio.
• If the patient's hematocrit exceeds 55%, the volume of citrate in the collection tube must be adjusted.
  • Adjustment Information:

High Hematocrit Samples. Patients with elevated hematocrits have a relatively low amount of plasma for a given whole blood (collection) volume. This tends to increase the plasma citrate concentration effectively. If the patient has a known hematocrit >55%, the amount of citrate in the collection tube must be decreased according to the formula below. The equation in CLSI H21 is as follows: C = (1.85 x 10−3) (100−Hct) (Vblood) Where: C is the volume of citrate remaining in the tube. Hct is the patient's hematocrit. V is the volume of blood added to the evacuated tube.

• Example:

Patient hematocrit = 60% Total volume = 5 mL (standard citrated plasma collection tube volume) C = (0.00185) x (100-60) x 4.5 C = (0.00185) x 180 (or 40 x 4.5) C = 0.333

• Immediately following collection, mix sample thoroughly by gently inverting 8 - 10 times to prevent clotting

1. Spin
2. Taking care not to disturb the cells, transfer plasma to a Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp) , labelled as Na cit plasma
3. Freeze immediately

Screw-cap polypropylene frozen transport vial/tube - 4mL (LabCorp)

Frozen (strict) - 11 days

Ambient - NO

Refrigerated - NO

Enzyme-linked Immunosorbent Assay (ELISA)

0 - 27 IU/mL