• Anti-IgG will be performed.
  • Elutions, Antibody screen and Antibody identification are not automatic and would need to be a separate order

The direct antiglobulin test (DAT) is a screening test for antibodies present on an individual's red cells and is used to investigate hemolytic disease of the newborn (HDN)

Lavender (EDTA), 4mL

• Transfer 4.0 mL cord blood to EDTA lavender tube.
• Avoid contamination with Wharton's jelly
• Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting
• Any combination of the cord ABO, Rh and/or DAT testing can be perfomed off of one 4mL EDTA tube

  Labelling instructions:
  Must be identified as Cord blood

  Newborn born at Allina Health must include the following ID information on the specimen tube:

  1. Mother's LAST name followed by BB (for boy) or BG (for Girl) and Mother's first name.
  2. For Multiples births, after the BB or BG, provide an additional identifier in order of birth: A, B, C, etc.
  3. The collection date and time, and A# of the collector must be on the tube.
   
  For Reference Lab cord blood samples:
  1. Label with Baby's first and last name, MRN or date of birth, and identify as cord blood.
  2. The collection date and time and collector's initials must be on the tube.

Lavender (EDTA), 4 mL

Refrigerated - 3 days

Sample should be obtained no more than 24 hours prior to testing but may be used up to 3 days post collection. Testing of older specimens may show decreased antibody reactivity.

• Improper label (unlabeled or mislabeled)
• Hemolysis

Column-agglutination, agglutination

Negative