All IHC stains will include a positive control tissue

• Is used to identify helper T cells
• This antibody is expressed on most mature T-cell lymphomas (except aggressive NK cell leukemia, extranodal NK cell lymphoma, gamma delta T-cell lymphoma, subcutaneous panniculitis-like T-cell lymphoma and enteropathy-type T-cell lymphoma)
• CD4 can be seen on some T-lymphoblastic lymphomas
• This antibody is used in a panel with other T cell markers, to assess antigen expression relationships of benign and malignant T cell populations; unexpected expression and or loss of CD4 can occur in neoplasms
• CD4 is often used with CD8 to evaluate helper/suppressor relationships of T lymphocytes

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• The CD4 molecule interacts with the HLA class II during antigen recognition
• CD4 antibody reacts with the CD4 protein associated with the helper subset of T lymphocytes
• CD4 is present in the majority of thymocytes
• CD4 also is present on various monocytic cells, such as Langerhans' cells, and other dendritic cells

Staining pattern

• Cell surface membrane staining

References

1. Macon WR, et al: T-cell subset analysis of peripheral T-cell lymphomas by paraffin section immunohistology and correlation of CD4/CD8 results with flow cytometry. Am J Clin Pathol, 109:610-617, 1998.