The PLAC® test for Lp-PLA2 Activity is an enzyme assay for the in vitro quantitative determination of Lp-PLA2 (lipoprotein-associated phospholipase A2) activity in EDTA plasma and serum. Lp-PLA2 activity is to be used in conjunction with clinical evaluation and patient risk assessment as an aid in predicting risk of coronary heart disease (CHD) in patients with no prior history of cardiovascular events.

Gold serum separator (SST) tube

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection

Gold serum separator (SST) tube

Red serum vial/tube - 5 mL

Lavender (EDTA), 4mL

Lavender:

Immediately after collection, mix tube thoroughly by gentle inversion, 8 - 10 times, to prevent clotting

Red:

1. Allow sample to clot
2. Spin
3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum, within two (2) hours of sample collection

Lavender:

1. Spin
2. Transfer plasma to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as EDTA plasma, within two (2) hours of sample collection

Transfer vial/tube with cap - 12mL (LabCorp)

Refrigerated (preferred) - 14 days

Ambient - 24 hours

Frozen - 18 months

• Hemolyzed samples
• Excessive turbidity
• Clots in samples

Spectrophotometric, enzymatic assay

Reduced risk:  <225 nmol/min/mL
Increased risk: ≥225 nmol/min/mL