Tomato (F25), IgE-4238
Test info
ImmunoCap
R45
Intended for diagnostic use as an aid in the clinical diagnosis of IgE mediated allergic disorders in conjunction with other clinical findings.
- Improper labels (unlabeled or mislabeled)
- Hemolysis (some procedures)
- Improper anticoagulant or ratio
- Delay in transport
- Improper storage temperature affecting results
- Improper container
- Leaking container resulting in compromised specimen
- Quantity not sufficient (QNS)
Specimen
- For more than one allergen, use this formula to calculate the amount of serum required:
50 µL X (# of allergens) + 250 µL = total amount serum needed
- Immediatley following collection, mix sample thoroughly by gently inverting 8 - 10, times to prevent clotting
- Allow sample to clot for a minimum of 30 minutes
- Spin within two (2) hours of sample collection
Gold serum separator (SST) tube
- Allow sample to clot
- Spin
- Transfer serum to a False bottom plasma/serum transport vial/tube (AHL), labelled as serum, within two (2) hours of sample collection
Ambient (preferred) - 3 days
Refrigerated - 7 days
Frozen (OK)
Performance
Fluorescence Enzyme Immunoassay (FEIA)
Clinical and Interpretive info
< 0.35 kU/L
Allergy class severity values
ImmunoCAP IGE Class Reference Values:
Traditionally, allergen tests have been reported with an associated class value of zero to 6, with Class 0 indicating an undetectable allergen level, and Class 6 indicating a strong positive allergen level.
The following chart correlates ImmunoCAP IgE allergen results in kU/L to the associated class, with a suggested interpretation. This chart is applicable to all allergen-specific tests at Allina Health Laboratory, which are performed exclusively on ImmunoCAP assays. Note that the Class Reference Values provided are specific for ImmunoCAP assays and may not be used to interpret allergy tests performed by other (non-ImmunoCAP) methods.
| Class | IgE kU/L | Interpretation |
| 0 | <0.35 | Undetectable |
| 1 | 0.35 - 0.70 | Equivocal |
| 2 | 0.71 - 3.50 | Positive |
| 3 | 3.51 - 17.50 | Positive |
| 4 | 17.51 - 50.00 | Strong positive |
| 5 | 50.01 - 100.00 | Strong positive |
| 6 | >100.00 | Strong positive |