Qualitative determination of IgM antibodies to Toxoplasma gondii (T. gondii) to aid in the presumptive diagnosis of acute or recent T. gondii infection, including in pregnant women. It is recommended that IgM testing be performed in conjunction with IgG testing. A positive IgM but negative IgG result should be viewed with suspicion; the patient should be retested two weeks after the first test. If the patient is pregnant and IgG/IgM positive, an IgG avidity test should be performed.

• Low levels of Toxoplasma IgM may persist for months to years after an initial infection.
• This test has not been cleared/approved by the FDA for blood/plasma donor screening.

Gold serum separator (SST) tube

Immediatley following collection, mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection

Gold serum separator (SST) tube

Red serum vial/tube, 5 mL

1. Allow sample to clot
2. Spin
3. Transfer serum to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as serum, within two (2) hours of sample collection

Transfer vial/tube with cap - 12mL (LabCorp)

Refrigerated (preferred) - 14 days

Ambient - 14 days

Frozen - 14 days

Freeze/thaw cycles: stable x4

• Hemolysis
• Lipemia
• Gross bacterial contamination

Chemiluminescent immunoassay (CLIA)

Negative: <8.0 AU/mL

Equivocal: 8.0 - 9.9 AU/mL

Positive:  >9.9 AU/mL