Determine abnormal T cell lymphoproliferative phenotype

Pathology orders only

Lavender (EDTA), 4mL

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

Lavender (EDTA), 4mL

Body fluid

• Collect 20 mL (minimum 5 mL) of body fluid and place in a Falcon vial/tube - 15mL
• Do not add anticoagulant or holding media
• The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
• Include representative slides (will be returned)

Bone marrow

• Collect 3 mL (minimum 1 mL) in a sodium heparinized (Na hep) syringe
• Specimens collected in EDTA are acceptable, but not preferred
• Transfer to an anticoagulant free sterile tube
• If unable to collect a bone marrow aspirate, a bone marrow core placed in RPMI with Hepes, Pen Strep and FBS is acceptable
• Submit representative slides (will be returned)

Cerebrospinal fluid (CSF)

• Collect 5 mL (minimum 1 mL) in a sterile vial/container.
• Do NOT add any anticoagulant or holding media
• The volume required is dependent upon the white blood cell (WBC) count. Smaller volumes are sufficient if the cell count is high
• Send representative slides (will be returned)

Tissue

• Immediately following collection, place the fine needle aspirate (FNA) or tissue in RPMI with Hepes/Pen strep/FBS -15 mL
• Send representative slides (will be returned)

Body fluid

Falcon vial/tube - 15mL

Bone marrow

Bone marrow transport vial/tube

Cerebrospinal fluid (CSF)

Sterile vial/container

Tissue

RPMI with Hepes/Pen strep/FBS -15 mL

Include previous Allina case number, if applicable.

EDTA whole blood

Ambient

Body fluid

Refrigerated

Bone marrow

Ambient

Cerebrospinal fluid (CSF)

Refrigerated

Tissue

Refrigerated

• Improper label (unlabeled or mislabeled)
• Wrong container (anticoagulant or solution)
• Tissues submitted in saline or B+ fixative are not acceptable and will be rejected
• Improper blood/anticoagulant ratio
• Delay in transport
• Improper storage
• Inappropriate timing of collection
• Interfering substances
• Leaking container

Flow cytometry

Tubes and Markers:

Tubes:

T1, T2, T3*

* T3 tube reflexed for an additional charge, only if immature or lymphoblastic features are detected in the T1 and/or T2 tubes

Markers:

CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD16, CD19, CD45, CD56, CD57, HLA-DR, TCRαβ, TCRγδ, TRBC1

* If T3 tube is reflexed, additional markers include: CD1a, CD3(cytoplasmic), CD34, TdT(nuclear))