• Work-up of individuals with autoimmune neutropenia
• Work-up of individuals having febrile nonhemolytic transfusion reactions
• Work-up for alloimmune neonatal neutropenia

This test is not useful for the diagnosis of neutropenia due to marrow suppression by drugs or tumors.

Red serum vial/tube, 5 mL

Immediately following collection, mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within two (2) hours of sample collection
3. Transfer the serum to a Screw cap transfer vial/tube (Mayo T914), labelled as serum

Screw cap transfer vial/tube (Mayo T914)

Refrigerated (preferred) - 30 days

Ambient - 7 days

Frozen - 1 year

• Specimen collected after a transfusion reaction
• SST or serum gel tubes
• Gross hemolysis

Flow Cytometry/Agglutination

Negative

Granulocyte antibodies are induced by pregnancy, prior transfusion, or transplants. These antibodies can cause neutropenia in various autoimmune disorders. Febrile nonhemolytic transfusion reactions and alloimmune neonatal neutropenia may also be caused by granulocyte associated antibodies, including anti-human leukocyte antigen antibodies.