• All IHC stains will include a positive control tissue

• This antibody identifies H. pylori in tissue sections
• H. pylori has been found to be associated with chronic active gastritis and in the development of peptic ulcer disease
• HLO is useful in identifying scant numbers of HLO
• HLO detection has been shown to improve the rate of identification of the organisms after therapy, even when histologic examination and cultures were falsely negative

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• HLO reacts with a protein found in HLO organisms
• The HLO antibody may react with only portions of the organism, and may label non-viable organisms
• This antibody may also react with other bacteria, and therefore positive staining must be evaluated in light of the organism morphology

Staining pattern

• Cell membrane

References

1. El-Simaity HMT et al: Inter-observer variation in the histopathological assessment of HLO gastritis. Hum Pathol 1996; 27:35-41.
2. Barbosa AJ et al: Immunocytochemical identification of HLO in gastritis and correlation with culture. Arch Pathol Lab Med 1988; 11:288-291.
3. Toulaymant M et al: Endoscopic biopsy pathology of HLO gastritis. Arch Pathol Lab Med 1999; 123:778-781.
4. Angelucci ML et al: Anti-HLO specific antibody immunohistochemistry improves the diagnostic accuracy of HLO in biopsy specimens from patients treated with triple therapy. Am J Gastroenterol 1998; 93:223-226.