All IHC stains will include a positive control tissue

• BCL6 staining is seen in follicular lymphomas, approximately 30% of diffuse large B cell lymphomas, Burkitt lymphomas, and nodular lymphocyte-predominant Hodgkin’s disease
• In normal tissues, BCL6 stains follicular center B-cells of the germinal centers, and some T-cells in the germinal centers; the staining pattern is reciprocal to that of Bcl-2
• BCL6 staining is not seen in B-CLL, hairy cell leukemia, and mantle and marginal-zone lymphomas

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

Tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• BCL6 is a protein expressed by germinal center B-lymphocytes and serves as a transcriptional regulatory protein
• BCL6 expression is seen in a variety of B cell lymphomas

 Staining patterns

• Nuclear staining

References

1. Falini B, et al: Bcl-6 protein expression in normal and neoplastic lymphoid tissues. Ann Oncol 8, Suppl, 2:101-104, 1997.
2. Flenghi L, et al: A specific monoclonal antibody detects expression of the BCL-6 protein in germinal center B cells. Am J Pathol 147:405-411, 1995.
3. Falini B, et al: Distinctive expression pattern of the BCL-6 protein in nodular lymphocyte predominance Hodgkin’s disease. Blood 87:465-71, 1996.