• All IHC stains will include a positive control tissue

• This antibody is typically used in conjunction with Pax 5
• Oct-2 is used to confirm B-cell lineage in CD20 negative B-cell lymphomas (Pax-5 positive and Oct-2 positive)
• This antibody can be used to confirm plasma cell lineage, including myeloma and plasmablastic lymphomas (Pax-5 negative but Oct-2 positive)
• Oct-2 can also be useful in phenotyping Hodgkin lymphoma (Pax-5 positive but Oct-2 negative)
• Expression of this antibody has also been reported in AML (30% in one study, in cases with myelomonocytic differentiation)

Submit a formalin-fixed, paraffin embedded tissue block

Formalin-fixed, paraffin embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• Oct-2 is a B-cell lymphocyte-specific transcription co-activator (associated with BOB.1), which has a role in germinal center formation and immunoglobulin productions (i.e. involved in the earliest stage of B-cell development)
• Oct-2 stains immunoglobulin secreting cells (B-cells and plasma cells)
• This antibody also recognizes neuronal cells

Staining pattern

• Nuclear staining

References

1. Ponzoni et al: New transcription factors in diagnostic hematopathology. Adv Anat Pathol. 2007 Jan;14(1):25-35. Review.
2. Gibson SE et al: Expression of the B cell-associated transcription factors PAX5, OCT-2, and BOB.1 in acute myeloid leukemia: associations with B-cell antigen expression and myelomonocytic maturation. Am J Clin Pathol. 2006 Dec;126(6):916-24.
3. Cue et al: Lineage determination of CD20- B-Cell neoplasms: an immunohistochemical study. Am J Clin Pathol. 2006 Oct;126(4):534-44.
4. McCune RC et al: Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin's and non-Hodgkin's lymphomas: a comparative study using high throughput tissue microarrays. Mod Pathol. 2006 Jul;19(7):1010-8. Epub 2006 Apr 28.
5. Brown P et al: The B-cell transcription factors BSAP, Oct-2, and BOB.1 and the pan-B-cell markers CD20, CD22, and CD79a are useful in the differential diagnosis of classic Hodgkin lymphoma. Am J Clin Pathol. 2003 Nov;120(5):767-77.