Detect vitamin B(6) deficiency

Lavender (EDTA), 4mL

Immediatley following collection, mix sample thoroughly by gentle inverting 8 - 10 times, to prevent clotting

1. Spin
2. Transfer plasma to a Amber transfer vial/tube - 12 mL (LabCorp), labelled as EDTA plasma

Amber transfer vial/tube - 12 mL (LabCorp)

Protect from light

Refrigerated (preferred) - 15 days

Frozen - 15 days

Freeze/thaw cycles - stable x6

Ambient - 3 days

• Anticoagulants other than EDTA
• Specimen not protected from light

Liquid chromatography-tandem mass spectrometry (LC/MS-MS)

3.4 − 65.2 μg/L

Deficiency:  <3.4

Marginal:       3.4 - 5.1

Adequate:    >5.1

Vitamin B6 deficiency can occur in individuals with a variety of genetic conditions including antiquitin deficiency,21 pyridox(am)ine-5'-phosphate oxidase (PNPO) deficiency22 and hyperprolinemia type II (pyrroline-5- carboxylate dehydrogenase deficiency. 23 Vitamin B6 levels can be decreased in malabsorption conditions including inflammatory disease of the small bowel and as a consequence of jejunoileal bypass. Several drugs, including oral contraceptive agents, levodopa, isoniazid, cycloserine, and pyrazinoic acid may cause B6 depletion. B6 levels may be decreased with pregnancy, lactation and alcoholism. Infants can develop deficiency when fed formula rendered B6 depleted by excessive heating.