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Extended RAS/RAF targeted NGS panel-Pathology add-on

Test info

Test name:

Extended RAS/RAF targeted NGS panel

Test number:

Pathology add-on

Abbreviation:

FOCUS

Alternate names:

Colorectal cancer NGS panel
Gastrointestinal cancer NGS panel
Hot spot mutations
Next Generation Sequencing
Oncomine focus assay

Tests included:

KRAS
NRAS
ERBB2
BRAF

Useful for:

Next Generation Sequencing (NGS) for the detection of alterations in solid tumors is critical for cancer care as these findings can direct the optimal use of targeted therapies for patients.

Limitations:

If blocks more than 5 years old are submitted for testing, extraction will be attempted, but if insufficient RNA/DNA is retrieved, testing will be cancelled

Specimen

Specimen type:

Tumor tissue

Transport container:

Formalin-fixed paraffin-embedded (FFPE) tissue block

Alternate transport container:

Cytology slide

Required forms/information:

Molecular Medicare billing request

Hospital clients submitting a request for this assay on an outpatient with Medicare should complete and submit a Molecular Medicare billing request form to notify us of the need for Allina Health Laboratory to bill insurance.

Transport and stability:

Ambient

Reason for rejection:

Improper label (unlabeled or mislabeled)
Improper blood/anticoagulant ratio
Interfering substances
Slides broken beyond repair

Performance

Performing lab:

AHL - Molecular Diagnostics: D

Days set up:

Varies

TAT:

10 business days

Method:

Next Generation Sequencing

Clinical and Interpretive info

Reference ranges:

No variations detected

Clinical information:

Fusion coverage table

Mutation coverage table

Billing

Preauthorization:

This test may require preauthorization from the insurance provider. Check the payer guidelines and, if needed, obtain the pre-authorization prior to sample collection.

CPT codes:

81275 - FOCUS KRAS CODONS 12 AND 13 NGS
81276 - FOCUS KRAS CODONS 61 AND 146
81479 - FOCUS ERBB2 NGS
81311 - FOCUS NRAS NGS
81210 - FOCUS BRAF NGS

Subject to medical necessity (Y/N):

Yes

Other billing information:

Medical necessity

Molecular Medicare billing request

Tracking

Date created:

12/07/2020

Revised date:

05/15/2024

Review date:

01/15/2024