All IHC stains will include a positive control tissue

• In normal prostate, CK HMW stains the basal layer of prostatic glands in a membranous/cytoplasmic pattern
• This antibody will not stain malignant prostate glands (due to the loss of the basal layer)

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• CK HMW stains epithelium that contains high-molecular weight cytokeratin

Staining pattern

• Cytoplasmic

References

1. Brimo F, Epstein JI: Immunohistochemical Pitfalls in Prostate Pathology. Hum Pathol 2012;43(3):313-324.
2. Hameed O, Humphrey PA: Immunohistochemistry in the Diagnosis of Minimal Prostate Cancer. Current Diagn Pathol 2006;12:279-291.
3. Martens MB, Keller JH: Routine Immunohistochemical Staining for High-Molecular Weight Cytokeratin 34-beta and alpha-Methylacyl CoA Racemase (P504S) in Postirradiation Prostate Biopsies. Mod Pathol 2006;19:287-290.
4. Varma M, Morgan M, Amin MB, et al: High Molecular Weight Cytokeratin Antibody (Clone 34beta12): a Sensitive Marker for Differentiation of High-Grade Invasive Urothelial Carcinoma from Prostate Cancer. Histopathol 2003;42:167-172.