All IHC stains will include a positive control tissue

• CD45 labels normal and neoplastic B and T lymphocytes; approximately 95% of B cell NHL and 85% of T cell NHL are positive for CD45
• Myeloid cells (and plasma cells) are weakly positive or negative for LCA
• Macrophages/histiocytes are usually negative for CD45
• Non-hematopoietic cells (benign and malignant) are negative for LCA
• CD45 reactivity may be weak or absent in plasmacytomas and anaplastic large cell lymphomas

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• LCA reacts with a family of 5 glycoproteins present on the surface of the majority of leukocytes

Staining pattern

• Cell surface membrane (sometimes also in cytoplasm)

References

1. Warnke RA et al: Diagnosis of human lymphoma with monoclonal anti-leukocyte antibodies. N Engl J Med 1983:309:1275-81.
2. Kurtin PJ et al: Leukocyte common antigen - a diagnostic discriminant between hematopoietic and non-hematopoietic neoplasma in paraffin sections using monoclonal antibodies: correlation with immunologic studies and ultrastructural localization. Hum Patho. 1985: 16:353.