• All IHC stains will include a positive control tissue

• Useful in identifying mesothelial cells (benign and malignant), although this marker cross-reacts with many tumors of other sites (such as from ovarian, lung, and GI tract)
• This marker is most useful in identifying papillary thyroid cancer; diffuse and intense membranous staining favors a papillary cancer
• Useful in battery with CK19 in discriminating papillary thyroid cancer from benign entities
• In a recent report ², HMBE1 staining was observed in 87 % of papillary thyroid cancers, including 43 of 49 (88%) classic papillary thyroid cancers and 25 of 29 (86%) follicular variants of papillary thyroid cancer
• CK19 staining has been reported in 96% of papillary thyroid cancers, including 100% of classic papillary thyroid cancer and in 26 of 29 cases (90%) of follicular variants of papillary thyroid cancer ²
• The co-expression of HBME1 and CK19 has been reported as 100% specific and 83% sensitive ²
• HMBE1 staining may rarely be seen in follicular adenomas (2 of 49 cases; 4%); this staining pattern is usually focal, rarely diffuse and strong. CK19 has been seen in 14% (7 of 29) of follicular adenomas
• In one study, 73% of lung adenocarcinomas are positive for this marker

Submit a formalin-fixed, paraffin-embedded tissue

Formalin-fixed, paraffin-embedded (FFPE) tissue block

FFPE tissue section mounted on a charged, unstained slide

Ambient (preferred)

• Unlabeled/mislabeled block
• Insufficient tissue
• Slides broken beyond repair

Immunohistochemical staining and microscopic examination

If requested, an interpretive report will be provided

Specifications

• HBME-1 reacts with an unknown antigen in the microvilli of mesothelial cells
• This antibody also reacts with a variety of benign and malignant epithelial cells
• HBME-1 will stain the majority of papillary thyroid cancers (including follicular variants); a negative result helps rule against a papillary cancer
• Non-specific staining for this marker can be seen in benign thyroid lesions and occasionally in follicular carcinomas

Staining pattern

• Membranous staining is most useful (cytoplasmic staining can also be seen)

References

1. Miettinen M et al: HBME-1, a monoclonal antibody useful in the differential diagnosis of mesothelioma, adenocarcinoma and soft-tissue and bone tumors. Appl Immunohistochem 1995; 3(2):115.
2. Scognamiglio T et al: Diagnostic usefulness of HBME1, Galactin-3, CK19, and CITED1 and evaluation of their expression in encapsulated lesions with questionable features of papillary thyroid carcinoma. Am J Clinc Pathol 2006; 126:700-708.
3. Nasr M et al: Immunohistochemical markers in diagnosis of papillary thyroid carcinoma: utility of HBME1 combined