This test is intended for use as an aid in the diagnosis of HIV-1 or HIV-2 infection, including acute or primary infection. Presence of HIV-1 or HIV-2 RNA in the plasma of patients without antibodies to HIV-1 or HIV-2 is indicative of acute or primary HIV-1 or HIV-2 infection. This assay may also be used as an additional test, when it is reactive, to confirm HIV-1 infection in an individual whose specimen is repeatedly reactive for HIV-1 and/or HIV-2 antibodies. This assay is not intended for use in donor testing.

Gold serum separator (SST) tube

Immediately following collection, thoroughly mix sample by gently inverting 5 times

1. Allow sample to clot for a minimum of 30 minutes
2. Spin within 24 hours of sample collection

Gold serum separator (SST) tube

Lavender (EDTA), 4mL

Immediatley following collection, mix sample by inverting 8 - 10 times to prevent clotting.

1. Spin within 24 hours of sample collection
2. Transfer plasma to a Transfer vial/tube with cap - 12mL (LabCorp), labelled as EDTA plasma

Transfer vial/tube with cap - 12mL (LabCorp)

Refrigerated (preferred) – 7 days

Ambient – 24 hours

Frozen – 6 weeks

• Specimen grossly hemolyzed
• Received outside of specimen and/or storage requirements

Real-Time Polymerase Chain Reaction (RT-PCR)

Non-reactive

Medical necessity